New Page 3

General operation
Balance, Top Loader	0.01g to 1500g
Balance, Analytical	0.1mg to 210g
Mechanical Balance	2-pan design 0.1g to 2000g
pH meter	--------------
Analytical Water System	Resistivity > 18.0 mΩ-cm TOC Less than 10 ppb	back to top
Storage - for generic biomaterial, chemical and reagent
Refrigerator	4ºC
Freezer	-20ºC
Freezer (BIEN/EVNG)*	-85ºC	back to top
Sterilization
Autoclave	Capacity: 53 Liter 60ºC to 132ºC 0-1.9Kgf/sq. cm(0-186.3kPa)	back to top
Heating - for agarose / water / reagent
Microwave Oven	--------------
Magnetic Stirring Hot Plate	100 – 1000 rpm 18 x 18 cm top plate	back to top
Drying
Oven 1 - for glassware / labware (BIEN/EVNG)*	30ºC to 200ºC, 161 Liter
Oven 2	30ºC to 250ºC, 108 Liter
Vacuum Oven	Capacity: 22 Liter 5ºC to 200ºC max. load: 35kg	back to top
Incubation - DNA denaturation, Medium preparation, Restriction digestion
Dry Bath	1.5ml tube, 20 places ambient up to 110ºC
Water Bath	5 Litre 5ºC above ambient up to 100ºC
Thermomixer	1.5 ml tube, 24 places slide 75 x 25 mm, 4 places 13ºC below room temp. to 99ºC 300 – 1400 rpm
Multi-Therm Shaker	0.5/1.5 ml tube 4 to 100ºC 200 – 1500 rpm	back to top
Liquid Handling
Auto-pipette p2, p20, p200, p1000	--------------
Auto-pipette 5ml, 10ml	--------------
Auto-pipette Aid	--------------
Repeater Pipette	--------------
Multi-channel Pipette	8-channel	back to top
Aspiration
Vacuum pump	Vacuum: 30mbar Bath volume: 4.4 Liter	back to top
Centrifugation - DNA precipitation, Cell harvesting
Personal Microcentrifuge	1.5/ 2ml tube, 6 places 6200 rpm
Bench-top Centrifuge	1.5 / 2ml tube, 24 places ambient temperature max. speed 13,200 rpm
Refrigerated Centrifuge	1.5 / 2ml tube, 15ml conical tube, 50ml conical tube, 250ml bottle, microplate -9ºC to 40ºC fixed angle: max. speed 14,000 rpm swinging bucket: max. speed 4,000 rpm
More centrifuges are available at BioCRF		back to top
Sample Mixing - Continuous mixing of samples, Preparation of reagents
Vortex	Touch Mode or Continuous Mode
Roto-Shake (BIEN/EVNG)*	Rotating: 5-35 rpm Rocking: 10-70 cycles/min Rocking Angle: + 10 degree from horizontal Platform Workload: 10 lb. (4.5 kg) 203 x 343 mm platform
Mixer	1 - 100 rpm 360° vertical rotation Tilting (reciprocation) Vibration
Orbital Shaker	0 – 800 rpm
Magnetic Stirring Hot Plate	100 – 1000 rpm 18 x 18 cm top plate
Thermomixer	1.5 ml tube, 24 places slide 75 x 25 mm, 4 places 13ºC below room temp. to 99ºC 300 – 1400 rpm
Multi-Therm Shaker	0.5/1.5 ml tube 4 to 100ºC 200 – 1500 rpm	back to top
Bacteria and Yeast Culture
Incubator	5ºC above ambient up to 99.9ºC
Shaking Water Bath	5ºC above ambient up to 99.9ºC 20-200 rpm
Environmental Shaker	5ºC above ambient up to 60ºC 40 – 400 rpm	back to top
Tissue Culture/Cell Biology
CO2 incubator	--------------
Cryogenic Storage Tank	Capacity: 111 Liter 4 racks 10 boxes/rack
Inverted Microscope CKX41	Phase contrast
Patch-clamp system at BioCRF
Electroporation system at BioCRF
Flow cytometer at BioCRF	back to top
Microscope
Inverted Microscope CKX41	Phase contrast
Upright Biological Microscope BX41	With fluorescent accessories and CCD camera
Upright Biological Microscope Ni-U	With fluorescent accessories and CCD camera
Inverted Microscope Ti2-E	With fluorescent accessories, motorized stage and CCD camera
More Microscopes available at BioCRF		back to top
Cell disruption
Sonifier cell disruptor	400 Watt Continuous or timed (0-15 minutes) operation Pulsed mode Process range from 1ml to over 500 ml
Grinding Mill	Speed: 20,000rpm Capacity: 250ml	back to top
Ultrasonic - Cleaning of slides / chips
Ultrasonic Water Bath (BIEN/EVNG)*	5.5 Litre Tank size: 290(L)x150(W)x150(H) mm 1 – 69ºC Frequency: 42 kHz	back to top
DNA Transformation
Micropulser Electroporator	output voltage: 200 – 3000V Max output current: 100amp
Gene Pulser for Eukaryotic System	Output Waveform Voltage: Exponential decay or square wave 10–3,000 V Capacitance: 10–500 V, 25–3,275 µF in 25 µF increments 500–3000 V, 10, 25, 50 µF Sample resistance: 20 ohm minimum at 10–2,500 V 600 ohm minimum at 2,500–3,000 V Square-wave timing: 10–500 V: 0.05–10 ms duration with 0.05 ms increments, 10–100 ms duration with 1 ms increments, 1–10 pulses, 0.1–10 sec interval 500–3,000 V: 0.05–5 ms duration with 0.05 ms increments, 1–2 pulses, 5 sec minimum interval
Electroporation system at BioCRF		back to top
PCR
Thermal Cycler Veriti	0.2ml PCR tube, 96 places or 96 well plate 4 – 99ºC
Thermal Cycler 9700	0.2ml PCR tube, 96 places or 96 well plate 4 – 99ºC
Thermal Cycler Proflex	0.2ml PCR tube, 3 x 32-well, 0.2 mL 0-100ºC
Real Time PCR System	0.2ml PCR tube 96 well plate single excitation, 4 emission filters and CCD camera 4 – 100ºC
Real Time PCR System	0.2ml PCR tube 96 well plate 6 filtered LEDs excitation, 6 filtered photodiodes detection Range of excitation and emission: 450-730nm 4 – 100ºC
Real-time PCR at BioCRF		back to top
Concentration
Vacuum Concentrator - for DNA/RNA/Proteins	1.5 / 2ml tube, 15ml tube
Rotary Evaporator	Vertical Condenser Rotation speed: 20-270rpm Bath temp: 20ºC to 180ºC Rate of evaporation: 1000H2O/h With diaphragm vacuum pump	back to top
Agarose Gel Electrophoresis
Gel Tank	Gel tray size: 7 x 8 cm 9 x 11 cm 12 x 14 cm Running voltage: 20 – 150 V
Power Supply	Voltage range: 10 – 300 V Current range: 5 – 500 mM Power Max. : 80 W	back to top
Polyacrylamide Gel Electrophoresis and Blotting
Eletrophoresis and Blotting unit 1: Includes 3 rectangular glass plates, 3 notched plates, 2 gel modules, lid, lower buffer chamber, 2 each 1.0-mm-thick 10-well combs and 1.0-mm-thick spacer sets.	Gel size: 8.3 x 9.7 cm
Eletrophoresis unit 2: Includes 5 combs, 5 sets of glass plates, 5 each 1.0 mm thickness 10-well combs; 2 casting stands, 4 casting frames, sample loading guide, electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam	Gel size: 7.5 x 10 cm
Eletrophoresis and Blotting unit 3: Includes 5 combs, 5 sets of glass plates, 5 each 1.0 mm thickness 10-well combs; casting stand, casting frames, sample loading guide, electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam	Gel size: 7.5 x 10 cm
Transblot Turbo	Max. 4 mini or 2 midi gels
Power Supply	Voltage range: 5 – 300 V Current range: 10 – 400 mM Power Max. : 80 W	back to top
Digital Imaging - Normalization quantitative analysis, Densitometry, Colony/Cell counting, Gel documentation
Gel Documentation System	CCD resolution: 1,360 x 1,024 (H x V) Pixel density: 12-bit (4,096 gray levels) Excitation source: 254, 302, 365 nm, and white light	back to top
Spectrophotometry - DNA/RNA quantitation, Protein determination, Cell culture OD600
Biophotometer	Xenon flash lamp 230, 260, 280, 320, 562, 595 nm band width: 5nm at 230 to 320nm 7nm at 562 to 595nm
Nanovue	Pulsed xenon lamp Sample size 0.5 μl to 5 μl Wavelength range 200–1100 nm (scanning 200–950 nm) Photometric range 0 to 125 A(10 mm pathlength equivalence)
Qubit 3	Dynamic range 5 orders of magnitude Ex Blue 430-495 nm Ex Red 600-645 nm Em Green 510-580 nm Em Red 665-720 nm Manual
Spectrophotometer (BIEN/EVNG)*	Deuterium/tungsten lamp 190-1100 nm in 0.1 nm data steps band width: <1.8nm scanning speed: 6200 nm/min	back to top


Microplate Reading
Microplate Reader	Fluorescence intensity (both top and bottom reading) Time-resolved fluorescence Luminescence Absorbance (VIS/UV)	back to top
Plasma Cleaning
Plasma Cleaner	6" diameter x 6" long chamber	back to top
Nanoparticle Analysis
qNano	Sample size 40μl Paritcle Size: 50nm-10μm concentration range 10⁵- 10¹² particles per mL	back to top
Freeze Drying
Freeze Dryer *Freeze Dryer User Registration Form	Collector Temperature: -105°C Ice Holding Capacity: 4.5 L Vacuum Pump Displacement Required: 86 L/Min. or larger displacement	back to top

* (BIEN/EVNG): shared equipment with Environmental Engineering