General operation

 

Balance, Top Loader

0.01g to 1500g

 
 

Balance, Analytical

0.1mg to 210g

 
 

Mechanical Balance

2-pan design

0.1g to 2000g

 
 

pH meter

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Analytical Water System

Resistivity > 18.0 mΩ-cm

TOC Less than 10 ppb

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Storage - for generic  biomaterial,  chemical and reagent

 
 

Refrigerator

4ºC

 
 

Freezer

-20ºC

 
 

Freezer (BIEN/EVNG)*

-85ºC

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Sterilization

 

Autoclave

Capacity: 53 Liter

60ºC to 132ºC

0-1.9Kgf/sq. cm(0-186.3kPa)

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Heating - for agarose / water / reagent

 
 

Microwave Oven

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Magnetic Stirring Hot Plate

100 – 1000 rpm

18 x 18 cm top plate

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Drying

 

Oven 1 - for glassware / labware (BIEN/EVNG)*

30ºC to 200ºC, 161 Liter

 

 

Oven 2

30ºC to 250ºC, 108 Liter

 
 

Vacuum Oven

Capacity: 22 Liter

5ºC to 200ºC

max. load: 35kg

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Incubation - DNA denaturation, Medium preparation, Restriction digestion

 
 

Dry Bath

1.5ml tube, 20 places

ambient up to 110ºC

 
 

Water Bath

5 Litre

5ºC above ambient up to 100ºC

 
 

Thermomixer

1.5 ml tube, 24 places

 slide 75 x 25 mm, 4 places

13ºC below room temp. to 99ºC

300 – 1400 rpm

 
  Multi-Therm Shaker

0.5/1.5 ml tube

 4 to 100ºC

200 – 1500 rpm

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Centrifugation - DNA precipitation, Cell harvesting

 

Personal Microcentrifuge

1.5/ 2ml tube, 6 places

6200 rpm

 
 

Bench-top Centrifuge

1.5 / 2ml tube, 24 places

ambient temperature

max. speed 13,200 rpm

 
 

Refrigerated Centrifuge

1.5 / 2ml tube, 15ml conical tube,

50ml conical tube, 250ml bottle, microplate

-9ºC to 40ºC

fixed angle: max. speed 14,000 rpm

swinging bucket: max. speed 4,000 rpm

 
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Sample Mixing - Continuous mixing of samples, Preparation of reagents

 
 

Vortex

Touch Mode or Continuous Mode

 
 

Roto-Shake (BIEN/EVNG)*

Rotating: 5-35 rpm

Rocking: 10-70 cycles/min

Rocking Angle: + 10 degree from horizontal

Platform Workload: 10 lb. (4.5 kg)

203 x 343 mm platform

 
  Mixer

1 - 100 rpm

360° vertical rotation

Tilting (reciprocation)

Vibration

 
 

Orbital Shaker

0 – 800 rpm

 
 

Magnetic Stirring Hot Plate

100 – 1000 rpm

18 x 18 cm top plate

 
 

Thermomixer

1.5 ml tube, 24 places

slide 75 x 25 mm, 4 places

13ºC below room temp. to 99ºC

300 – 1400 rpm

 
  Multi-Therm Shaker

0.5/1.5 ml tube

 4 to 100ºC

200 – 1500 rpm

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Bacteria and Yeast Culture

 

Incubator

5ºC above ambient up to 99.9ºC

 
 

Environmental Shaker ZQZY-108C

 

4-60ºC

10-300 rpm

 
 

Environmental Shaker IS-971

 

Amb. +5 to 60 ºC

10-300 rpm

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Tissue Culture/Cell Biology

 

CO2 incubator

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Cryogenic Storage Tank

Capacity: 111 Liter

4 racks

10 boxes/rack

 
 

Inverted Microscope CKX41

Phase contrast

 

Microscope

 

Inverted Microscope CKX41

Phase contrast

 
Upright Biological Microscope Ni-U With fluorescent accessories and CCD camera
  Inverted Microscope Ti2-E With fluorescent accessories, motorized stage and CCD camera  

Cell disruption

 

Sonifier cell disruptor

400 Watt

Continuous or timed (0-15 minutes) operation

Pulsed mode

Process range from 1ml to over 500 ml

 
 

Grinding Mill

Speed: 20,000rpm

Capacity: 250ml

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Ultrasonic - Cleaning of slides / chips

 
 

Ultrasonic Water Bath (BIEN/EVNG)*

5.5 Litre

Tank size: 290(L)x150(W)x150(H) mm

1 – 69ºC

Frequency: 42 kHz

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DNA Transformation

 

Micropulser Electroporator

output voltage: 200 – 3000V

Max output current: 100amp

 
Gene Pulser for Eukaryotic System

Output  Waveform Voltage:
Exponential decay or square wave
10–3,000 V

 

Capacitance:

10–500 V, 25–3,275 µF in 25 µF increments
500–3000 V, 10, 25, 50 µF

 

Sample resistance:

20 ohm minimum at 10–2,500 V
600 ohm minimum at 2,500–3,000 V

 

Square-wave timing:

10–500 V: 0.05–10 ms duration with 0.05 ms increments, 10–100 ms duration with 1 ms increments, 1–10 pulses, 0.1–10 sec interval
500–3,000 V: 0.05–5 ms duration with 0.05 ms increments, 1–2 pulses, 5 sec minimum interval

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PCR

 

Thermal Cycler Veriti

0.2ml PCR tube, 96 places or

96 well plate

4 – 99ºC

 
  Thermal Cycler Proflex 0.2ml PCR tube,
3 x 32-well, 0.2 mL
0-100ºC
 
  Real Time PCR System

0.2ml PCR tube

96 well plate

6 filtered LEDs excitation, 6 filtered photodiodes detection

Range of excitation and emission: 450-730nm

4 – 100ºC

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Concentration

 

Vacuum Concentrator -  for DNA/RNA/Proteins

1.5 / 2ml tube, 15ml tube

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Agarose Gel Electrophoresis

 

Gel Tank

Gel tray size: 7 x 8 cm

                       9 x 11 cm

                       12 x 14 cm

Running voltage: 20 – 150 V

 
 

Power Supply

Voltage range: 10 – 300 V

Current range: 5 – 500 mM

Power Max. : 80 W

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Polyacrylamide Gel Electrophoresis and Blotting

 

Eletrophoresis and Blotting unit 1:

Includes 3 rectangular glass plates, 3 notched plates, 2 gel modules, lid, lower buffer chamber, 2 each 1.0-mm-thick 10-well combs and

1.0-mm-thick spacer sets.

 

Gel size: 8.3 x 9.7 cm

 

 

 
 

Eletrophoresis unit 2:

Includes 5 combs, 5 sets of glass plates, 5 each 1.0 mm thickness 10-well combs; 2 casting stands, 4 casting frames, sample loading guide, electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam  

 

Gel size: 7.5 x 10 cm

 

 

 
 

Eletrophoresis and Blotting unit 3:

Includes 5 combs, 5 sets of glass plates, 5 each 1.0 mm thickness 10-well combs; casting stand, casting frames, sample loading guide, electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam  

 

Gel size: 7.5 x 10 cm  
Transblot Turbo Max. 4 mini or 2 midi gels
 

Power Supply

Voltage range: 5 – 300 V

Current range: 10 – 400 mM

Power Max. : 80 W

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Digital Imaging Normalization quantitative analysis, Densitometry, Colony/Cell counting, Gel documentation

 
 

Gel Documentation System

CCD resolution:  1,360 x 1,024 (H x V)

Pixel density: 12-bit (4,096 gray levels)

Excitation source: 254, 302, 365 nm, and white light

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Spectrophotometry DNA/RNA quantitation, Protein determination, Cell culture OD600

 
 

Biophotometer

Xenon flash lamp

230, 260, 280, 320, 562, 595 nm

band width: 5nm at 230 to 320nm

                    7nm at 562 to 595nm

 
 

Nanovue

Pulsed xenon lamp

Sample size 0.5 μl to 5 μl

Wavelength range 200–1100 nm (scanning 200–950 nm)

Photometric range 0 to 125 A(10 mm pathlength equivalence)

 
 

Qubit 3


Dynamic range 5 orders of magnitude
Ex Blue 430-495 nm
Ex Red 600-645 nm
Em Green 510-580 nm
Em Red 665-720 nm
Manual
 
 

Spectrophotometer (BIEN/EVNG)*

Deuterium/tungsten lamp

190-1100 nm in 0.1 nm data steps

band width: <1.8nm

scanning speed: 6200 nm/min

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  Microplate Reading    
  Microplate Reader Fluorescence intensity (both top and bottom reading)

Time-resolved fluorescence

Luminescence

Absorbance (VIS/UV)

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  Plasma Cleaning    
  Plasma Cleaner 6" diameter x 6" long chamber back to top
  Nanoparticle Analysis    
qNano

Sample size 40μl

Paritcle Size: 50nm-10μm

concentration range 10- 1012 particles per mL

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  Freeze Drying    
  Freeze Dryer

*Freeze Dryer User Registration Form
  • Collector Temperature: -105°C
  • Ice Holding Capacity: 4.5 L
  • Vacuum Pump Displacement Required: 86 L/Min. or larger displacement
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      * (BIEN/EVNG): shared equipment with Environmental Engineering

 

    More equipments are available at BioCRF, please visit their website.