Two-fold serial dilutions of a crude E.
coli protein extract and samples of purified recombinant green fluorescent
protein were separated on a 12% polyacrylamide SDS gel run on miniVE. Gel
was stained with Coomassie™ Blue.
Detection of E. coli GroEL following
Western transfer in miniVE Blot Module. Two-fold serial dilutions of a crude
protein extract were separated on a 12% polyacrylamide SDS gel run on miniVE.
Proteins were transferred to Hybond™-P membrane using miniVE Blot Module.
GroEL was detected using an anti-GroEL antibody, ECL Plus™ Western Blotting
Detection System, and Hyperfilm™ ECL™.
Extra Length and Compatible Dimensions
miniVE handles gel cassettes up to 10 × 10.5 cm (W × H), which increases the
separation length by more than 30% over conventional 10 × 8 cm gels. In
addition, miniVE is compatible with the glass plates, combs, and spacers of
SE 260 Mini-Vertical units. For gradient gels, mini-Vertical Gel Casters can
produce up to four identical gels at one time.
The Right Power Supplies
EPS 301 is the power supply of choice for miniVE System. With ratings of 300
V and 400 mA, it is capable of both running and blotting gels. For faster
transfers when blotting four gels at a time, use the EPS 2A200 for its
higher current capability.
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