Thermal cycling system Peltier-based, 96-well block

Sample Ramp Rate +/-1.1°C/sec

Peak Block Ramp Rate 1.6°C/sec

Temperature Range 4°C – 100°C

Temperature Accuracy ±0.25°C of setpoint/display temperature, measured at 3 minutes after clock start.

Temperature Uniformity ±0.50°C, 30 seconds after clock start

Optical system Single excitation, four emission filters, and CCD camera

Calibrated Dyes at Installation SYBRR Green I, FAM, VIC, JOE, NED, TAMRA, ROX

Passive Reference Dyes ROX or any calibrated dye. Selection of no passive reference is optional

Data Collection Data collected in all 4 filters for all wells regardless of plate setup. Plate setup may be altered after run completes.

Quantitative PCR run time < 1 hour 50 minutes

Supported Volumes 20 – 100 μL

Supported Consumables ‧ Standard Optical 96-well plates

‧ 8-strip 0.2mL tubes

‧ 0.2mL tubes

‧ Optical Adhesive Covers

‧ Optical Flat Caps